第六外显子编码高亲和力的DNA结合域,通过同源重组,用一个包含PGK-neo和胸腺嘧啶激酶选择标记的构建进行同源插入,使得LoxP序列环绕。随后,通过将靶向的ES细胞转染含Cre表达质粒,短暂转染去除了PGK-neo-thymidine kinase标记,使得第六外显子两侧留有LoxP位点。(来源:J:93481)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
4
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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