Tg(ZP2)2Dean

The transgene consists of a 16.3 kb human genomic DNA fragment containing all 19 exons of the zona pellucida 2 gene and 2.3 kb of 5' flanking DNA. Northern blot analysis and in situ hybridization of ovarian tissue demonstrated oocyte-specific transgene expression. Immunoblot analysis revealed coexpression of mouse and human ZP2 in oocytes. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein. (J:93482)