Cldn9^nmf329

This mutation was discovered in an ENU mutagenesis screen. A T-to-C single nucelotide substitution in codon 35 is predicted to change the 35th amino acid of the encoded protein from phenylalanine (Phe) to leucine (Leu), located in the first predicted extracellular loop. Immunohistochemistry experiments with a polyclonal antibody that recognizes the unique C-terminus of the protein showed that the mutant protein is expressed in a spatiotemporal manner similar to the normal protein. (J:152154)