一种新霉素抗性基因座替换了编码基因大部分催化区域的2-6号外显子。对新生仔鼠(POSTNATAL DAY 0)脑组织的 Western blot 检测并未在纯合子小鼠中发现蛋白。(来源:J:92666)
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A neomycin resistance cassette replaced exons 2-6, which encode most of the catalytic region of the gene. Western blot analysis of brain extracts from postnatal day 0 did not detect protein in homozygous mice. (J:92666)