同源重组被用来删除大约3.2kb的序列,包括ATG起始密码子。皮肤组织的 Western blot 和免疫沉淀实验确认了突变小鼠中没有蛋白质表达。(来源:J:92500)
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Homologous recombination was used to delete approximately 3.2 kb of sequence including the ATG start codon. Western blot and immunoprecipitation of skin sections confirmed that no protein was expressed in mutant mice. (J:92500)