设计了一种靶向载体,用于替换exon 3和exon 4的部分序列,替换为PGK-beta-geo筛选 cassette。Southern blot结果显示目标细胞被正确地标记。RT-PCR结果显示表达完全被抑制。(来源:J:92161)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129X1/SvJ
Targeted
载体插入引起的破坏
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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