插入了带有博泰戈基因剪接 cassette 的元件到内皮2号染色体。PCR结果显示小鼠中发生了重组。北印迹实验显示转录被阻断,而 Western 印迹则证实了突变体大脑中完全没有蛋白质(来源:J:92302)
查看原文 参与反馈
A floxed Betageo cassette was inserted into intron 2. PCR confirmed recombination in mice. Northern blot demonstrated that transcription was abolished and Western blot indicated a complete absence of protein in mutant brains. (J:92302)