在ATG后面9个碱基的位置插入了EGFP-neo。Southern blot和RT-PCR的结果表明转导成功,且突变细胞中没有野生型的转录本。流式细胞术显示内源性蛋白的表面表达缺失。GFP表达与Klre1表达的丧失呈负相关。(来源:J:92262)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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