在与表达Cre重组酶的小鼠广泛杂交后,去除了1和2号外显子。PCR确认了在ES细胞和小鼠中发生了重组,并且 Western blot 显示突变的ES细胞中没有产生蛋白质。(来源:J:92282)
查看原文 参与反馈
Floxed exons 1 and 2 were excised following upon mating with mice ubiquitously expressing Cre recombinase. PCR confirmed recombination in ES cells and mice and Western blot indicated no protein was produced in mutant ES cells. (J:92282)