在3-5号外显子的周围插入了LoxP位点。插入了带有FRT标记的无启动子的β-半乳糖苷酶-新霉素基因座,位于5号内含子。与EIIa-Cre小鼠杂交后,去除了包含3-5号外显子的含LoxP的区域,保留了FRT标记的β-geo。对突变体的免疫组化分析证实了内源性蛋白的缺失。对突变新生儿小肠组织的 Western blot 也证实了蛋白的不存在。(来源:J:92210)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae
Targeted
插入,基因内删除
--
1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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