这个靶向构建包含了基因的2-4号外显子以及由loxP序列包裹的诺卡因抗性基因。克隆通过Cre质粒转染。筛选出的ES细胞系显示了2-4号外显子和诺卡因抗性基因的缺失,然后用于微注射。通过PCR、Southern blot和原位杂交确认了这一突变(来源:J:92100)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129X1/SvJ
Targeted
插入,基因内删除
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1
1
24

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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