整个编码区域被Cre-PGKpuro cassette替换。RT-PCR结果显示野生型细胞中没有内源性转录物,突变体中有Cre。 Western blot证实突变体大脑匀浆中未检测到内源性蛋白。(来源:J:91814)
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The entire coding region was replaced with a Cre-PGKpuro cassette. RT-PCR indicated lack of endogenous transcript and presence of Cre in mutants. Western blot confirmed that no endogenous protein was present in mutant brain lysates. (J:91814)