将含有三个GAL4结合位点(UAS)和一个带有floxed ura3和neomycin抗性基因座的靶向构建插入到了CTCF位点下游的DMR区域。短暂的Cre表达剪切掉了ura3和neo基因座。敲入操作并未干扰H19的非编码RNA表达。(来源:J:91713)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count