使用靶向载体在内源性起始密码子下游每个框架位置插入了终止密码子。在内源调节序列下游放置了IRSV-EGFP,并插入了条件性Flp-floxed PGKneo。Southern blot确认了重组。原位杂交显示突变大脑纹状体区域的mRNA几乎检测不到。使用反义RNA探针进行的原位杂交显示EGFP mRNA在突变体中被检测到。纹状体蛋白质提取物的Western blot结果显示了存在于野生型和敲除体中的潜在非特异性带。因此,突变基因产生了可检测不到的Rasd2蛋白。(来源:J:91597)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
--
1
--
13

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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