整个开放阅读框通过同源重组被一个带有floxed neo cassette的替换,通过RT-PCR和 Western blot分析证实了纯合突变体中RNA和蛋白质表达的缺失。(来源:J:91621, J:91622)
查看原文 参与反馈
The entire open reading frame was replaced with a floxed neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of RNA and protein expression in homozygous mutants. (J:91621, J:91622)