在包含预测的PARP催化区域的第3和4外显子被替换为PGK-neo cassette。Northern分析未检测到转录本,而 Western blot结果显示突变的MEFs中没有蛋白质。(来源:J:91080)
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Exons 3 and 4, which include the predicted PARP catalytic domain, were replaced with a PGK-neo cassette. Northern analysis showed no transcripts and Western blot indicated lack of protein in mutant MEFs. (J:91080)