在经同源重组,通过LoxP位点在第2外显子和第二个内含子后的PGK-neo cassette周围插入了序列。 Western blot分析证实了突变等位基因(J:91025)产生了蛋白质表达。 (Source: None)
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LoxP sites were inserted around exon 2 and after a PGK-neo cassette in intron 2 via homologous recombination. Western blot analysis confirmed protein expression from the mutant allele (J:91025)