Rspo2^ftls

This mutation was caused by the insertion of 6 to 8 copies of a transgenic construct containing the chloramphenicol acetyltransferase (CAT) gene adjoined to a rat H+/K+ ATPase promoter. Approximately 7 kb of genomic DNA was deleted at the insertion site. This insertion/deletion was shown to be located in intron 3 of the Rspo2 gene. No exons of the gene were deleted. Analysis of the transcript by RT-PCR, northern and in situ hybridization analysis showed that a smaller transcript is expressed from this allele that results from the splicing of exon 3 to a cryptic splice site in the inserted transgene. RT-PCR analysis indicates that a very low level of full-length transcript is expressed; however, no full length transcript is detectable by in-situ hybridization. This allele is described as a severe hypomorph. (J:83881, J:131960)