在转基因中,一个新霉素抗性基因 cassette 替换了 ex1 的起始密码子和下游序列。对同源突变胚胎成纤维细胞的 Western blot 分析未检测到蛋白质。(来源:J:47298)
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A neomycin selection cassette replaced the start codon and downstream sequence from exon 1. Protein was undetected by Western blot analysis of homozygous mutant embryonic fibroblasts. (J:47298)