Kit^tm3.1Bsm

Cre-mediated recombination removed the floxed neo in Kittm3Bsm, leaving a single loxP site in intron 9 and the tyrosine 567 to phenylalanine mutation. Flow cytometry indicated that receptor levels were reduced to 50-60% of normal levels, and Rnase protection assay showed that transcripts are reduced to 50-60% of normal levels, putatively indicating that the remaining lox site and flanking sequences in intron 9 of the mutant affects RNA transcription and/or splicing. (J:90485)