Log In|Sign Up
中文
由于重组异常，原本设计用于在exon 2区域插入一个带有floxed neo cassette的靶向载体，错误地插入到了intron 3。内源性exon 2保持不变，随后是intron 1的重复区域、载体衍生的exon 2被floxed neo cassette打断，以及intron 2的重复部分。测序结果显示，插入点处额外检测到24bp的未知来源序列。这导致了Npc2 mRNA的严重剪接错误，通过RT-PCR和western blotting分别检测到的野生型水平正确剪接的mRNA和蛋白分别只有大约4%。(来源：J:89617)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
