Tg(ACTB-NOTCH1)1Shn

This transgene consists of the chicken beta-actin promoter followed by a loxP flanked stop, the human NOTCH1 intracellular domain (amino acids 1762-2304), and an SV40 late polyadenylation signal. Cre mediated recombination removes the stop and allows expression of the NOTCH1 gene. Only single site insertions were used to generate mice. This line carries 10 - 20 copies of the transgene and protein is detectable by western blot analysis after cre mediated recombination. (J:90392)