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A point mutation was introduced into exon 17 causing a valine to isoleucine change at amino acid residue 642 (V642I) together with a floxed neomycin resistance gene in intron 17. The neomycin gene was subsequently excised from correctly targeted clones by transient cre expression. The mutation was confirmed in mice by genomic DNA sequencing. (J:90296, J:90561)