Aldh1a3^tm1Pcn

A floxed neomycin cassette was inserted in the antisense orientation downstream of exon 9 while a third loxP site was inserted upstream of exon 8. Subsequently, the neomycin resistance gene was excised by transient transfection of properly target ES cells with a cre-expressing plasmid and leaving exons 8 and 9 which code for the catalytic site flanked by loxP sites. (J:90261)