在exon 2中插入了一个带有三重终止密码子的敲除neo cassette, Western blot结果显示突变细胞中没有正常蛋白表达。(来源:J:89403)
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A floxed neo cassette, as well as three stop codons in three different frames, was inserted into exon 2. Western blot indicated absence of normal protein in mutant cells. (J:89403)