通过同源重组,将启动子区域和exon 1替换了neo cassette。 Western blot结果显示,纯合无基因突变小鼠中没有检测到蛋白。(来源:J:89499)
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The promoter region and exon 1 were replaced with a neo cassette via homologous recombination. Western blot analysis showed the absence of protein in homozygous null mice. (J:89499)