在Cre介导的重组通过去除一个含floxed neo的neo基因后,exon 10包含了氨基酸替换为F的Y340、Y341和E343K。 Western blot确认了突变等位基因的蛋白质表达。(来源:J:89619)
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Following the excision of a floxed neo by Cre-mediated recombination, exon 10 contained the amino substitutions Y340F, Y341F, and E343K. Western blot confirmed protein expression of the mutant allele. (J:89619)