在编码序列下游插入了一个IRES-GFP-pA-neo cassette。流式细胞术、RT-PCR和 Western blot的结果表明,突变小鼠中存在GFP表达、Runx1转录物和相应蛋白。(来源:J:89055)
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An IRES-GFP-pA-neo cassette was inserted downstream of the coding region. Flow cytometry, RT-PCR and Western blot indicated presence of GFP expression, Runx1 transcript, and protein, respectively, in mutant mice. (J:89055)