Ccnf^tm1.1Sje

Floxed exon 2 and neo cassette are removed following transient Cre-mediated recombination, leaving a single loxP site. Removal of exon 2 putatively generates a null mutation by preventing the encoding of F box as well as the creation of a frameshift mutation and a premature stop codon after encoding 5 amino acids and 39 amino acids, respectively. PCR confirmed recombination. (J:89060)