通过Flp介导的重组在Dnaja3tm1Jdl中移除了带有frt内切座的neo cassette,留下了一个被修饰的exon 2。突变型MEFs的 Western blot结果显示,蛋白表达与野生型一致(来源:J:89091)。
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Flp-mediated recombination removed the frt-flanked neo cassette in Dnaja3tm1Jdl, leaving floxed exon 2. Western blot of mutant MEFs demonstrated protein expression was that of wild-type. (J:89091)