在5' UTR通过暂时的Cre表达去除了一个floxed诺卡因抗性基因后,保留了被floxed的2-5号外显子。同源突变体大脑的 Western blot 分析显示,蛋白质水平与野生型相同。(来源:J:89288)
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Floxed exons 2-5 remained following the excision of a floxed neomycin resistance gene in the 5' UTR by transient Cre expression. Western blot analysis of homozygous mutant brains indicated protein was present at wild-type levels. (J:89288)