Cul7^tm1Jdec

A targeting vector containing a FRT site flanked NEO cassette and a loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 4. Mice carrying this allele, Cul7tm2.1, were crossed to mice expressing FLP recombinase to remove the FRT site flanked neo selection cassette. Subsequent cre excision removed exons 2 through 4 leaving a single loxP site. (J:85161)