Cre介导的重组会去除Ephb1tm1Sor中2-5号含floxed标签的外显子,留下一个由PGK-neo-bpA序列环绕的FRT座点。胚胎匀浆的 Western blot结果显示内源性蛋白已不存在。(来源:J:89008, J:143845)
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Cre-mediated recombination removes the floxed exons 2-5 in Ephb1tm1Sor, leaving a PGK-neo-bpA cassette flanked by FRT sites. Western blot of embryo lysates confirmed absence of the endogenous protein. (J:89008, J:143845)