同源重组被用来将exon 1的编码区域替换为GFPpA连接到一个含floxed neo cassette的构造。随后,通过cre介导的重组移除了neo cassette,留下GFPpA和一个loxp位点。在9.5-14.5期发育中胚层的异种鼠中,GFP荧光水平与已知的内源蛋白表达水平相符。(来源:J:88653)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入,基因内删除
--
1
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26

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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