同源重组被用来将exon 1的编码区域替换为GFPpA连接到一个含floxed neo cassette的构造。随后,通过cre介导的重组移除了neo cassette,留下GFPpA和一个loxp位点。在9.5-14.5期发育中胚层的异种鼠中,GFP荧光水平与已知的内源蛋白表达水平相符。(来源:J:88653)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count