从4到7号外显子被通过同源重组替换为了诺卡因选择标记。通过RT-PCR和 Western blot分析,我们确认了纯合突变MEFs中没有表达。(来源:J:88125)
查看原文 参与反馈
Exons 4 through 7 were replaced with a neomycin selection cassette via homologous recombination. The absence of expression in homozygous mutant MEFs was confirmed by RT-PCR and Western blot analysis. (J:88125)