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中文
在5'非翻译区插入了一个包含neo抗性基因和胸苷激酶基因的敲除和frt框标记选择 cassette。随后在翻译终止密码子下游161bp的位置插入了一个loxP座。通过转染表达flp重组酶的载体，对靶向的ES细胞成功地去除了neo和胸苷激酶基因，Northern blot和Western blot的检测证实了这两个转录本和蛋白产物的缺失。(来源：J:88126)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
