将人类的GLI3基因cDNA和一个下游的loxP引导的诺姆选择座插入到Gli2内源性基因的首编码外显子中。插入的cDNA被上游的FLAG表位和下游的mouse Gli2 3' UTR,以及三个SV40 polyA信号的重复序列所包夹。研究表明,这个插入等位基因的GLI3表达水平低于去除了诺姆座的衍生等位基因Gli2tm5.1(GLI3)Alj(来源:J:87948)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
--
1
10
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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