Trp53^tm2Glo

An arginine to proline substitution was generated at codon 172 by an engineered point mutation introduced at coding nucleotide 515 (c.515G>C). p.R172P, equivalent to p.R175P in humans, is associated with human tumors defective in apoptosis. A single loxP site was left in intron 4 after the excision of a loxP site flanked neomycin resistance gene cassette via Cre-mediated recombination. Western blot analysis of homozygous MEFs detected full length protein at levels that exceeded those of full length protein in wild-type MEFs. (J:87501)