在第13号外显子中插入了一段PGK-抗药性基因。通过 Western blot 分析,无法检测到蛋白质产品,而纯合小鼠的细胞内天冬氨酸转氨酶活性也消失了。(来源:J:87889)
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A PGK-neomycin resistance gene was inserted into exon 13. Protein product was undetectable by Western blot analysis and cytosolic aconitase activity was lost in homozygous mice. (J:87889)