在第二外显子插入了新霉素抗性基因座。通过分子杂交试验和RT-PCR确认了mRNA缺失。通过免疫学方法和 Western blot 分析也证实了蛋白的缺失。(来源:J:87615)
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A neomycin resistance cassette was inserted in exon 2. Absence of mRNA was confirmed by hybridization assay and by RT-PCR. Absence of protein was demonstrated immunologically and by Western blot analysis. (J:87615)