Pou2f1^tm1Shrp

Exon 3 was replaced with a floxed neo cassette. The deleted exon is common to all three known isoforms and does not overlap with Cd247, a locus that overlaps a distal portion of the Pou2f1 locus. Mutant transcript, in which exon 2 aberrantly splices to exon 4a, was identified by RT-PCR analysis of homozygous mutant MEFs. The aberrant splicing results in a frameshift mutation which consequently generates a nonsense mutation and precludes the translation of two-thirds of the mature protein including the DNA-binding domain. Supershift analysis of DNA binding assays using nuclear extracts from homozygous cells detected very low levels of protien, thus this is a severely hypomorphic allele. (J:87664)