Pnpla6^tm1Mos

A cassette containing IRES-lacZ and neo was inserted at codon 397, upstream of the carboxyl-terminal catalytic domain. Western blot analysis of lysates from cultured cells indicated an absence of protein of in homozygous mutant embyros. A 50% reduction of esterase activity in tissue homogenates obtained from heterozygous mutant mice indicated functional ablation. (J:87675)