Dsc1^tm1Pko

Intron 16 and exon 17 were deleted and the 3' end of exon 16 was fused to the 3' UTR. A floxed PGK-neomycin cassette was also inserted 0.23kb downstrean of exon 16. The neomycin cassette was subsequently excised by transient transfection of properly targeted ES cells with a Cre expression plasmid. RT-PCR analysis indicated that an aberrant mRNA was produced which retained intron 15. A truncated protein product is expected rather than gene disruption. (J:87593)