整个编码区域被插入了通过同源重组插入的诺美西林选择座。脾和胸腺组织的 Western 印迹分析显示,纯合突变小鼠中没有检测到蛋白质。(来源:J:87535)
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The entire coding region was replaced with a neomycin selection cassette inserted by homologous recombination. Western blot analysis of splenic and thymic lysates indicated an absence of protein in homozygous mutant mice. (J:87535)