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Exons 11 and 12, which includes the sequence for the intracellular region, were replaced with a complex sequence including a cDNA fragment encoding only the first half of the cytoplasmic signaling domain, an SV40 polyadenylation site, and a floxed PGK-neomycin cassette. Western blot analysis demonstrated that the resulting protein product had been modified to a size consistent with a 60 amino acid deletion. (J:87416)