Tg(Rho)1Wbae

The transgenic construct contained a 15 kb genomic fragment encompassing the mouse rhodopsin gene. 3 substitution mutations were engineered in exon 1 (V20G, P23H, and P27L). 6 kb of upstream sequence and 3 kb of downstream sequence were included in the construct. Northern blot analysis showed that both the inserted mutant rhodopsin transcript and the wild-type transcript are present in transgenic mice, indicating that the site of integration is not at the endogenous rhodopsin locus. (J:12791)