Emx1^tm1(cre)Krj

An IRES-cre gene followed by an FRT flanked PGK-neomycin gene was inserted 120 bp 3' of the stop codon. The neomycin gene was later removed in vivo by crossing to an Flp recombinase expressing transgenic strain. The protein product of this gene was produced in normal quantities as confirmed by Western blot analysis. Cre recombinase expression was characterized by crossing to a Rosa26 strain. (J:78098)