在 intron 2 中插入了floxed的诺卡因抗性基因,同时在exon 1中插入了loxP位点。构建出的产物是一个表达量减少80%的弱变种,蛋白质产物也相应减少。通过将针对的ES细胞短暂转染一个cre表达载体,以去除诺卡因但保留exon 1和2,且这两个区域被loxP位点环绕,也得到了一个表现出相似表型的弱变种。(来源:J:86857)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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