在第7个内含子插入了条件性PGK-新霉素抗性基因,同时在第7内含子75bp处额外放置了一个loxP位点,接着通过Cre介导的重组,正确靶向的ES细胞中去除了第8外显子和新霉素基因。预测这种转录产物在第7和9外显子之间的剪接点将产生框移突变。(来源:J:86827)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
插入,基因内删除
--
1
3
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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