一段1.4kb的基因组片段,包含了近端启动子和exon 1,被替换了抗氨苄青霉素的选择性标记。通过同源突变 CNS组织的 Western blot 检测,未发现蛋白质表达(来源:J:86663)。
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A 1.4 kb genomic fragment, containing the proximal promoter and exon 1, was replaced with a neomycin selection cassette. Protein was undetected by Western blot analysis of homozygous mutant CNS tissue. (J:86663)